Vol. 5, Issue 2, Part A (2023)

The objective of the current study was to investigate several biological functions of an Agave attenuata leaf methanol extract. There were 25 chemicals identified by GC-MS in the n-hexane fraction of the extract, with mono-2-ethylhexyl phthalate (10.31%), 1, 2-benzenedicarboxylic acid (5.03%), n-docosane (5.45%), and eicosane (5.04%) serving as the main constituents. The total phenolic content in the leaves was 11.521-39.35 GAE mg/100 g, while the total flavonoid content was 42.35-304.8 CE mg/100g. Moderate antibacterial properties were seen in the extract and several of its components. By measuring DPPH radical scavenging activity and inhibiting lipid peroxidation experiments, leaf extract and fractions also showed good antioxidant ability. Hemolytic effects of the plant were found to range from 2.01% to 3.50%. This plant shows potential as a source of natural antioxidants and functional food nutraceuticals, according to the study's findings.