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International Journal of Pharmacy and Pharmaceutical Science

Vol. 6, Issue 1, Part A (2024)

Comparative study of Anabaena azolla using three different nutrient media

Author(s):

Ugbaje Blessing Ojoche, Adams Osiekafore Adinoyi, Mathias Akoji G, Yusuf Abel, Okoliko Victor Micheal, Akoh Stephen, Priscilla Onuche Unekuojo and Obajemu Babatunde Samuel

Abstract:

Increasing demand for protein, especially animal-based proteins and the large amounts of protein feed inputs required for production, has largely driven the research on Anabaena azolla (AZ) as an animal feed. Overall, AZ appears to be a prospective protein source in fish production, as well as aquaculture. The Proximate, Mineral, Phytochemical and Vitamin composition of cultured AZ was determined. The cultured samples were grouped into four as follows T1 was the Control, and group T2, T3, and T4 was treated every 24 hours with 2 mg/L urea and 40 mg Sodium bicarbonate. In the proximate analysis the T4 group has significantly high percentage moisture content (1.73%) followed by T3, T2 and T1 respectively when compared at (p≤ 0.05). The mineral composition shows significant increase in the Na composition of the T4 (778.00 Mg/Kg) and T3 (775.21 Mg/Kg) (groups followed by T2 730.30 Mg/Kg) group at (p≤ 0.05). The K component of T4 (303.40 Mg/Kg) group is significantly higher than other treated groups. There is no significant difference in the composition of T1 (159.20 Mg/Kg) and T2 (177.40) Mg composition at (p≤ 0.05). The Ca content is significantly high in increasing other (T1 (304.20 Mg/Kg) to T4 (330.50 Mg/Kg) groups) at (p≤ 0.05). There is no significant difference in the Fe and P component of T3 (17.60 Mg/Kg & 410.49 Mg/Kg) and T4 (18.00 Mg/Kg & 427.50 Mg/Kg) as well as T2 (15.30Mg & 390.50 Mg/Kg) and T1 (14.00 Mg/Kg & 348.15 Mg/Kg) at (p≤ 0.05). The Zn and Cu composition of T4 (5.15 Mg/Kg & 0.88 Mg/Kg) is significantly high followed by T3, (4.77 Mg/Kg & 0.75 Mg/Kg) T2, (4.19 Mg/Kg & 0.53 Mg/Kg) and T1 (3.88 Mg/Kg & 0.39 Mg/Kg) at (p≤ 0.05) respectively. The Phyto-analysis reveals no significant difference in T2 to T4 groups. The T4 (80.40 Mg of GAE/g of sample) has the highest Total phenol properties followed by T3, 53.25 Mg of GAE/g of sample) T2 (36.49 Mg of GAE/g of sample) and T1 (15.45 Mg of GAE/g of sample) at (p≤ 0.05). T3 (10.58 Mg of GAE/g of sample) and T4 (12.90 Mg of GAE/g of sample) group significantly poses high Flavonoid component at (p≤ 0.05). The T4 (2.06 Mg of DE/g of sample) group significantly poses high Saponin content followed by T3 (1.89 Mg of DE/g of sample) and T2 (1.52 Mg of DE/g of sample) at (p≤ 0.05). There is significantly high percentage alkaloid in T4. (0.11% Alkaloid). The cardiac glycoside value is significantly high in T4 (2.25 Cardiac glycoside Mg Securida side /g of sample) groups at (p≤ 0.05). The Vitamin compositions shows no significant difference in vitamin A component in T1 (85.40) and T2 (99.16) and also, T3 (128.70) and T4 (133.00) respectively at (p≤ 0.05). There is no significant difference in Vitamin C composition of T4 (15.70) and T3 (14.90) as well as T2 (12.74) and T1 (12.60) respectively at (p≤ 0.05). The AZ when treated with other substances existing in either organic or inorganic nature is proven to be rich in essential nutrients required for normal growth of fishes and other aquatic animals. Hence, the T4 should be considered as the most potent sample treated with AZ. As a result of these properties, it is required that the biochemical and in vivo antioxidant capacity of these treatments should be further investigated.

Pages: 20-23  |  101 Views  36 Downloads


International Journal of Pharmacy and Pharmaceutical Science
How to cite this article:
Ugbaje Blessing Ojoche, Adams Osiekafore Adinoyi, Mathias Akoji G, Yusuf Abel, Okoliko Victor Micheal, Akoh Stephen, Priscilla Onuche Unekuojo and Obajemu Babatunde Samuel. Comparative study of Anabaena azolla using three different nutrient media. Int. J. Pharm. Pharm. Sci. 2024;6(1):20-23. DOI: 10.33545/26647222.2024.v6.i1a.79
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