Vol. 6, Issue 2, Part A (2024)

Development of UV method for simultaneous estimation of Olmesartan Medoxomil development was done by Q-Absorbance ratio method and area under curve method and stability indicating studies using methanol as solvent. Most of the studies are not well authenticated and not cross validated by any methodology. Here we have made an attempt to develop a simple, specific, accurate, precise and reproducible method for the simultaneous estimation of Olmesartan Medoxomil (OLM) and Hydrochlorothiazide (HTZ) in combined dosage form by UV spectrophotometric method, the method includes area under curve method (Method I) and Q- absorbance Ratio method (Method II). The wavelengths are 243 nm and 272 nm λmax of both the drugs were prepared for Method I, and for Q- absorbance Ratio method (Method II) 252 nm an iso absorptive wavelength and 272 nm were selected for estimation of Olmesartan Medoxomil and Hydrochlorothiazide respectively and the two drugs are follow Beer’s law over the concentration range of 1-6 μg/ml. The % recoveries of the both the drugs were identified to be nearly 100 % representing the accuracy of the proposed methods. LOD and LOQ values of OLM was found to be 0.146, 0.201, 0.136, 0.422, 0.407, 0.486 at different wavelengths 272 nm, 252 nm, 243 nm respectively and LOD and LOQ values of HTZ were found to be 0.135, 0.182, 0.133, 0.410, 0.55, 0.405 at 272 nm, 252 nm, 243 nm respectively. Validation of the proposed methods was preformed out for its accuracy, precision, specificity and ruggedness as per ICH guidelines. The proposed methods successfully validated in routine work for determination of Hydrochlorothiazide and Olmesartan medoxomil in combined dosage form.