Vol. 7, Issue 2, Part A (2025)

Salbutamol sulphate, a widely prescribed β₂-adrenergic receptor agonist, is extensively used for the treatment of respiratory diseases such as asthma and chronic obstructive pulmonary disease (COPD). Accurate and sensitive analytical techniques for its quantification are critical for pharmaceutical quality control, clinical monitoring, doping control, and environmental analysis. Over the past three decades, numerous analytical methods have been developed and optimized, ranging from simple spectrophotometric approaches to sophisticated chromatographic and electrochemical techniques. This review systematically compiles and evaluates the analytical methodologies reported for the determination of salbutamol sulphate in various matrices including pharmaceuticals, biological fluids, and environmental samples. The methods discussed include UV-Visible spectrophotometry, spectrofluorimetry, high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), capillary electrophoresis (CE), electrochemical sensors, and mass spectrometry (LC-MS/MS). Each method is examined for sensitivity, selectivity, precision, accuracy, and applicability, highlighting key author contributions and validation parameters per ICH guidelines. Two comprehensive tables summarize the comparative analytical performance of spectrophotometric and electrochemical methods. The review concludes by discussing current trends and future perspectives, emphasizing the role of nanomaterials, green analytical chemistry, and advances in chiral separation techniques for salbutamol analysis.