Vol. 7, Issue 2, Part D (2025)

A high-performance liquid chromatography method was developed to estimate Losartan and Chlorthalidone together from the drug product they are combined. The method is simple, precise, accurate, and reproducible. The separation was done using a Shimadzu SPD-10A (250×4.6, 5µm) column with an inner diameter of 3.9 mm, C18 type. The mobile phase was a mix of acetonitrile and water in the ratio of 85:15 (v/v), and the flow rate was set at 1.2 ml/minute. The column was maintained at normal room temperature. The detector wavelength was 238 nm. The retention times for Losartan and Chlorthalidone were 1.76 minutes and 2.71 minutes, respectively, with a total run time of 10 minutes. The method was tested for different aspects such as system suitability, specificity, linearity, precision, accuracy, robustness, and ruggedness. Low percentages of RSD values showed the method is accurate and precise, making it suitable for the quantification of both drugs in drug products and biological samples. This method is suitable for use in routine quality control testing of final drug products without any interference from other components