Vol. 7, Issue 2, Part F (2025)

This Research investigated the anti-inflammatory activity of aqueous ethanolic Ocimum gratissimum root extract through In silico molecular docking and In-vitro egg albumin denaturation assay study. The fresh plant root part was carefully collected, properly identified, and authenticated, was thoroughly washed, shade-dried, and then coarsely ground into a fine powder. The dried powder was subjected to hot extraction method using the Soxhlet extraction technique with aqueous: ethanol (2:8) as the solvent. Subsequently, the presence of phytochemical constituents was determined using standard qualitative chemical tests. Further molecular docking was done using 1CX2 protein carried by TLC for better binding score compounds. Later In-vitro protein denaturation assay study was done in which desi egg was taken and from it 1% egg albumin solution was prepared and subjected to Anti-inflammation activity by using the extract in different concentrations (100, 200, 300, 400, 500mcg/ml) with that of standard drug Diclofenac sodium (100, 200, 300, 400, 500mcg/mll). The absorbance was recorded by using UV double beam spectrophotometer and compared, % Inhibition were calculated. The results showed that 300,400,500mcg/ml of extract showed significant better effect than Std drug indicating enhanced inhibition of protein denaturation and, therefore, stronger anti-inflammatory potential. whereas others (100,200mcg/ml) too showed anti-inflammatory effects but less than standard, reflecting comparatively weaker anti-inflammatory activity. These findings suggest that the extract’s anti-inflammatory effect increases with concentration implying a dose dependent response. Thus, the study concludes that the aq ethanolic extract of Ocimum gratissimum exhibits anti-inflammatory activity.