Vol. 7, Issue 2, Part I (2025)

The current study reports the antioxidant, anti-inflammatory and anti-diabetic activities of Cicer arietinum leaf aqueous extract (CALAE). CALAE showed antioxidant properties by scavenging DPPH free radicals in a dose-dependent manner with an IC₅₀ value of 39.33µg/µl. The free radical quenching ability of CALAE was further evaluated using sodium nitrite and diclofenac induced oxidative stress in the RBCs and rat model. Interestingly, CALAE normalized stress markers such as Lipid peroxidation, protein carbonyl content, Total thiol, Superoxide Dismutase and Catalase in a dose-dependent manner in both RBCs and tissue homogenate of rats. Furthermore, the toxicity study of Cicer arietinum plant extract was done by direct hemolytic activity; data revealed that it did not lyse red blood cells; hence it is non-toxic in nature. Furthermore, CALAE inhibited denaturation of both egg and bovine serum albumin in a dose-dependent manner, suggesting its anti-inflammatory property. Interestingly, CALAE inhibition of the α-amylase and α-glucosidase activity showed the anti-diabetic property. In conclusion, CALAE showed, antioxidant, anti-inflammatory and anti-diabetic properties.